Dados do Trabalho

TÍTULO

INTERSPECIFIC CHROMOSOME PAINTING IN PLECTUROCEBUS CALIGATUS (PITHECIIDAE, PRIMATES)

Resumo

<p>The New World primates commonly known as titi monkeys are characterized by a complex taxonomy and great karyotypic diversity. These primates, previously considered as a single genus, <em>Callicebus</em>, have recently been divided into three genera: <em>Callicebus</em>, <em>Cheracebus</em> and <em>Plecturocebus</em>. <em>Plecturocebus</em> is subdivided into the groups <em>P. donacophilus</em> and <em>P. moloch</em>. The latter is composed of 16 species, including <em>P. caligatus</em>. A female specimen of <em>P. caligatus</em> from the Centro de Primatologia do Rio de Janeiro (CPRJ) was cytogenetically analyzed. The chromosome preparations obtained from lymphocyte and fibroblast cultures were used for GTG- and CBG-banding and for fluorescent <em>in situ</em> hybridization (FISH) with individual human chromosome probes (HSA). Our specimen displayed a diploid number (2n) 48 and a fundamental number (NF) 60. Eight human autosomes (HSA 6, 9, 11, 14, 17, 18, 20 e 21) and the X chromosome were conserved in <em>P. caligatus</em>. Three of these chromosomes, HSA 6, 20 and X, hybridized to a whole <em>P. caligatus</em> chromosome, whereas HSA 9, 11, 14, 17, 18 and 21 were associated with other autosomes. Multiple hybridization signals were observed for the remaining human autosomes. <em>Plecturocebus caligatus</em> exhibited the associations HSA 2/16, 3/21, 5/7, 8/18, 10/16 and 14/15 present in the putative Platyrrhini ancestral karyotype. We also detected the association HSA 2/16 with an inversion and the associations HSA 22/2/22, 7/15, 10/11, which are present in the putative Callicebinae ancestral karyotype. Other associations detected were HSA13/21, which is a synapomorphy of <em>P. moloch</em>, and the association HSA 13/17, which suggests a link between the <em>P. moloch</em> group and <em>Callicebus</em>.</p>

Palavras-chave

<p>Platyrrhini, Fluorescent in situ hybridization (FISH), Karyotipic evolution, Titi monkeys</p>

Financiamento

<p>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).</p>

Área

Genética