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OSTEOGENIC POTENTIAL OF MULTICELLULAR SPHEROIDS CONSTITUTED BY HUMAN BURSA SUBACROMIALIS-DERIVED MESENCHYMAL STEM CELLS.
INTRODUCTION: Regenerative Medicine is a branch of medicine that has markedly advanced in recent years. Achievements are mainly related to use of mesenchymal stem cells (MSCs), associated or not with biological supports and/or bioactive molecules, with intention of improving the efficiency of tissue regeneration process. Although several studies have shown the potential of MSC therapy, especially on the bone healing process, there is a need to expand the knowledge in order to enable its clinical utilizaty in an effective and safe way. Parameters as obtaining the source of MSC and culture procedures in cell expansion phase are known to be able to influence the cellular niche and, consequently, the therapeutic potential of this technique.
Objetivos - Metodologia - Resultados - Discussão dos Resultados/Objectives - Methodology - Results - Discussion of Results/Objetivos - Metodología - Resultados - Discusión de los resultados
OBJECTIVE: To analyze the osteogenic potential of multicellular spheroids from human bursa subacromialis-derived mesenchymal stem cells (hBSDMSC). METHODOLOGY: Fragments of the bursa subacromialis were extracted from a 48 years old volunteer by arthroscopic procedure and submitted to a process to isolate the mesenchymal cell contingent. Upon reaching third passage, the mesenchymal profile of cells was determined by adipogenic and osteogenic differentiation and flow cytometry. Multicellular spheroids were constituted by non-adherent surface culture technique. After 5 days of culture, spheroids started to be cultivated in osteogenic medium for an additional period of 14 days. The osteogenic differentiation potential of hBSDMSC in spheroids was determined by alizarin red staining technique. RESULTS: Characterization procedures confirmed the mesenchymal profile of cells isolated from human bursa subacromialis. After culturing on a non-adherent surface, cells constituted multicellular spheroids, which reached an average diameter of 200 μm after x time. Alizarin red staining demonstrated the osteogenic differentiation potential of hBSDMSC cultured as multicellular spheroids.
Considerações Finais/Final considerations/Consideraciones finales
hBSDMSC, cultured as multicellular spheroids in an "in vitro" expansion phase, constitute a promising alternative for research and clinical applications in bone regenerative medicine strategies.
Palavras-chave/Key words/Palabras clave
Mesenchymal stem cells, Regenerative medicine, Cell therapy, Multicellular spheroids
Mesenchymal stem cells/adultas
ANDRE CAPALDO AMARAL, Guilherme Erlo, Sandro Baraldi Moreira, Renata Aquino de Carvalho, Janaina Andréa Dernowsek, Rodrigo Alvarenga Rezende, Jorge Vicente Lopes da Silva