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Título/Title/Titulo

Bone Biofabrication From Human Adipose Stem/Stromal Cell Spheroids Seeded Into PLA/CHA 3D Printed Scaffold

Introdução/Introduction/Introdución

New bone formation is desirable in a variety of pathological states and also in critical-size defects. Adipose-derived stromal/stem cells (ASCs) can be considered a promising cell source for tissue engineering because of its easy accessibility and abundance. We proposed a novel tissue engineering approach based on adult stem cells spheroids seeded into scaffolds.

Objetivos - Metodologia - Resultados - Discussão dos Resultados/Objectives - Methodology - Results - Discussion of Results/Objetivos - Metodología - Resultados - Discusión de los resultados

The aim of the study is to interact ASCs spheroids with a 3D printed composite scaffold of polylactic acid (PLA) and nanostructured carbonated hidroxiapatite (CHA). ASCs were harvested by a mechanical dissociation of human lipoaspirates obtained according to local research ethics committee. ASCs were seeded in a non-adhesive agarose hydrogel micromolded with 81 ressections and maintained in a medium containing ascorbic acid 50 µM, insulin 5 mg/mL, transferrin 5 mg/mL and selenium 5ug/mL (ITS) and human albumin 1.25 μg/mL. After 24 hours, one spheroid was formed in each resection. Then, spheroids were collected from the hydrogel, manually seeded on the surface of a PLA/CHA composite scaffold and maintained for one week in culture. The interaction of spheroids with the scaffold was evaluated by SEM. It was observed that migrating cells from spheroids spread over the scaffold surface. Furthermore, some spheroids have changed their morphology, interacting with a larger area of biomaterial. We also observed that cells from spheroids proliferated and produced extracellular matrix components over the biomaterial, improving cell adhesion with the scaffold surface.

Considerações Finais/Final considerations/Consideraciones finales

Therefore, it was possible to produce an engineered constructed from human ASCs spheroids seeded into PLA/CHA scaffolds for bone tissue engineering applications. Our perspective is to implant the engineered constructed into a calvaria defect model to evaluate bone regeneration in vivo.

Palavras-chave/Key words/Palabras clave

Bone, Spheroids, 3D scaffold, Tissue Engineering

Área

Tissue engineering

Autores

GABRIELA SOARES KRONEMBERGER, THIAGO NUNES PALHARES, ALEXANDRE MALTA ROSSI, JOSÉ MAURO GRANJEIRO, LEANDRA SANTOS BAPTISTA