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Bioglass 45S5: structural characterization and analysis of biocompatibility with adipose-derived mesenchymal stromal cells in vitro and in vivo
Bioactive glasses have potential applications in the field of regenerative medicine due to their bioactivity that permits interaction with both hard and soft tissues. In the same way, mesenchymal stromal cells (MSCs) have been experimentally tested as part of engineered constructs considering their self-renewal and multipotent capacities. However, to design an association, it is crucial to investigate the physical properties of bioglass 45S5, as well as its biocompatibility.
Objetivos - Metodologia - Resultados - Discussão dos Resultados/Objectives - Methodology - Results - Discussion of Results/Objetivos - Metodología - Resultados - Discusión de los resultados
Therefore, we investigated the structural short-range order of the stoichiometric 45S5, by obtaining its total structure factors (S(K)) and its radial distribution functions (RDF). The in vitro compatibility of human MSCs with 45S5 was verified by viability, morphometry and osteoinduction assays, and scanning electron (SEM) analysis. The compatibility outcome was verified through a subcutaneous implantation in a murine model by grafting the 45S5 as a scaffold for allogeneic MSCs.
Considerações Finais/Final considerations/Consideraciones finales
An increase of hydroxyapatite (HA) depositions around cells was observed. Cells showed satisfactory compatibility patterns when growing over 45S5 up to 90 days. The implant did not show any apparent toxicity for organs, or strong immunogenic reactions, and it was accompanied by a dense capsule formation around the graft. Our results indicate that MSCs can grow in the long term on the 45S5 while maintaining their characteristics. This fact, together with a non-toxicity to animals means that the 45S5 can be implemented in pre-clinical trials aiming MSC’s transplantation leading to further bone and tissue repair.
Palavras-chave/Key words/Palabras clave
bioglass 45S5; mesenchymal stromal cell; bioactivity; short range order; radial distribution functions
CRISTIANO RODRIGUES, Carla Zanatelli, Thais Casagrande Paim, Liliana Sous Naasani, Jéssica Gonçalves Azevedo, Márcia Rosângela Wink, Silvio Buchner