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Scaffold production of nanofibrous polycaprolactone and ascorbic acid 2-phosphate magnesium
Tissue engineering involves the search for biological substitute materials favorable for the development and regeneration of new tissue. Polycaprolactone (PCL) is one of the synthetic polymers of the first choice for this purpose. Ascorbic acid is a bioactive compound with very promising antioxidant functionality for use in medicine.
Objetivos - Metodologia - Resultados - Discussão dos Resultados/Objectives - Methodology - Results - Discussion of Results/Objetivos - Metodología - Resultados - Discusión de los resultados
The aim of this study has been to cultivate stem cells on PCL scaffolds with ascorbic acid 2-phosphate (ASAP). The stem cells were isolated from human teeth and characterized in the laboratory. To produce the scaffolds, PCL (MW=80,000) was dissolved in tetrahydrofuran:N, N-dimethyl formamide (7:3) to create a 10% (w/v) solution. The set-up to conduct the electrospinning included a high voltage of 19 kV, a collector to needle distance of 15 cm, and a constant flow rate of 1.14 mL/h. Scaffolds of PCL and PCL/ASAP were produced. The ASAP concentration was 25 µg/ml per scaffold. A total of 30,000 cells/well were seeded in tissue culture plates (TCP) used as a control group or on the scaffolds in a 24-well plate and the analysis was performed after 7 days. Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Statistical analyses were performed by ANOVA (P<0.05). A statistical difference was not found between the TCP, PCL and PCL/ASAP groups (p = 0.096). The average ± standard deviation (SD) values of absorbance obtained related to cell viability were: 0.16 ± 0.02 for the TCP, 0.20 ± 0.02 for the PCL) and 0.21 ± 0.03 for the PCL/ASAP).
Considerações Finais/Final considerations/Consideraciones finales
Therefore, both groups are non-cytotoxic for stem cells and are safe for tissue regeneration studies.
Acknowledgments: MCTIC, FINEP, CNPq, Stem Cell Research Institute (IPCT).
Palavras-chave/Key words/Palabras clave
Keywords: ascorbic acid, polycaprolactone, regenerative medicine, scaffold.
Scaffold (surface topology and softness)
RAFAELA HARTMANN KASPER, GABRIELA MORAES MACHADO, NATASHA MAURMANN, MYRIAN BREW, MARCOS DO COUTO , ANDRÉ WILTGEN, CÉLIA MAHL, CAREN SERRA BAVARESCO, PATRÍCIA PRANKE