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Título/Title/Titulo

THE SECRETOME OF ADIPOSE STEM CELLS HUMAN PROTECTS AGAINST GENOTOXICITY GENERATED BY OXIDATIVE STRESS

Introdução/Introduction/Introdución

Human life expectancy is increasing due to the quality of life improvement. Likewise, pathologies associated with aging are also growing and aggravating social and economic sectors. The aging process is related to the increase of damage in DNA whit loss of genetic integrity. The accumule of the stem cells damaged contributes to the progress of human aging. Adipose stem cells (hASC) secretome has cytoprotective effect and reduce damage associated with early cellular senescence.

Objetivos - Metodologia - Resultados - Discussão dos Resultados/Objectives - Methodology - Results - Discussion of Results/Objetivos - Metodología - Resultados - Discusión de los resultados

The objective of this research is to evaluate the effect of hASC-conditioned medium in the upkeep of human mesenchymal stem cell integrity genetic. Methods: To achieve our objective, in this research we analyzed nuclear anomaly by blockade of cytokinesis by cytochalasin B assay (CBMN) and immunodetection genotoxicity 8-Oxig.Our results showed a reduction of nucleocytoplasmic bridge and micronucleus, indicators of chromosomal instability and reduction of the presence of 8-Oxig, which suggests reduction of oxidative damages in the DNA in the groups treated with secretome of hASC.

Considerações Finais/Final considerations/Consideraciones finales

In conclusion, the secretome from hASC protects mesenchymal stem cells against genetic instability. Consequently, this secretome has the potential to preserve the stem cell status that is lost during human aging.

Palavras-chave/Key words/Palabras clave

secretome, hASC.

Área

Mesenchymal stem cells/adultas

Autores

CAMILA ACORDI DA SILVA, PRISCILLA BARROS DELBEN, Adriane Cristina Fagundes, ROGERIO S GOMES, ANDREA GONÇALVES TRENTIN