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INFLUENCE OF BIOACTIVE CERAMICS ON KERATINOCYTES’ VIABILITY
Bioactive ceramics (BC) are an important class of biomaterials used in tissue engineering because of their positive interactions with not only hard tissue (bone and teeth), but also soft tissue, such as skin.
Objetivos - Metodologia - Resultados - Discussão dos Resultados/Objectives - Methodology - Results - Discussion of Results/Objetivos - Metodología - Resultados - Discusión de los resultados
In this study, a BC of the SiO2–CaO–Na2O–P2O5 system was synthesized and tested regarding its influence on the viability of keratinocytes, which are a cellular type found in the outermost layer of the skin. For this purpose, the human cell lines HaCaT were seeded in 96-well tissue culture plates (TCPs) and treated with BC at concentrations of 0.5, 1, 1.5, and 2 mg/mL. Cell viability was then evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (MTT) after four days of cultivation. The mean and standard deviation values obtained from the normalized results of the cell viability (%) of the treated groups compared to the TCPs (used as control group) were 100.0 ± 13.9 % for TCP, 140.2 ± 24.2% (p<0,02) for 0.5 mg/mL, 235.0 ± 31.6 % (p<0,00) for 1 mg/mL, 232.8 ± 31.8% (p<0,00) for 1.5 mg/mL and 296.8 ± 37.2 % (p<0,00) for 2 mg/mL of the BC. The composition and crystallinity of bioceramics are aspects which make them capable of affecting the concentration of ions in the cell culture medium and, consequently, influencing the cell viability. By varying the concentration of the bioactive ceramics in the cell culture, it was therefore possible to favor HaCaT proliferation for a particular application of interest, such as wound healing and skin regeneration. Comparisons between the tested BC showed that the cell viability was dose-dependent, with the maximum evaluated (2 mg/mL) being statistically better than the others and with the “p” value below 0.005 in all cases. In conclusion, the tested BC can be used to promote cell viability, thereby being a useful tool in tissue engineering with further application in regenerative medicine.
Considerações Finais/Final considerations/Consideraciones finales
As a following step, evaluation of the material will be made with a view to creating a product for skin regeneration in vivo.
Acknowledgments: MCTIC, FINEP, CNPq and Stem Cell Research Institute (IPCT).
Palavras-chave/Key words/Palabras clave
Keywords: bioactive ceramics, biomaterials, tissue engineering, regeneration.
MARINA MORAES MATTARREDONA BRIAO, DANIELA PAVULACK, RENATO SIQUEIRA, NATASHA MAURMANN, EDGAR ZANOTTO, PATRICIA PRANKE