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Incorporation of the cobalt therapeutic ion into bioactive glass network and its implications for angiogenesis
Introduction: Bioactive glasses (BGs) are used mainly for the repair of bone tissues due to their high bioactivity and biocompatibility. BGs have a variable and highly flexible composition, allowing the incorporation of metallic ions with therapeutic effect, a propitious approach in the development of BGs with superior properties for tissue engineering. These ions have the advantage of being released exactly at the implant site by optimizing the therapeutic effect and reducing side effects in the patient as they are released in a controlled manner during the process of material degradation. Cobalt (Co) presents great potential for incorporation into the structure of bioactive glasses, since it may influence the formation of new blood vessels from the stabilization of the hypoxia induction factor (HIF) and vascular endothelial growth factor (VEGF), known to activate genes related to angiogenesis.
Objetivos - Metodologia - Resultados - Discussão dos Resultados/Objectives - Methodology - Results - Discussion of Results/Objetivos - Metodología - Resultados - Discusión de los resultados
Objective: In this context, this work aims to develop novel sol-gel bioactive glass compositions and evaluate the incorporation of cobalt on the glass network as a potential strategy to develop superior materials with sustained ion release for tissue engineering. Methodology: Microparticles of Co-containing bioactive glasses with up to 2,5% CoO was obtained and analyzed by ICP-OES assays, Endothelial Cell Growth Curve, MTT, Immunohistochemistry. Results and discussion: The composition of the microparticles of Co-containing bioactive glasses was confirmed by chemical analysis. The influence of the incorporation of cobalt in partial substitution to the CaO content in the structural and biological properties of the bioactive glasses was studied. The construction of the endothelial cell growth curve consisted of analyzing the progress of cell monolayer density over time. This analysis allowed to obtain estimated data of the folding time of the cell lines for use in the experiments of cellular proliferation and immunofluorescence. Endothelial cell in vitro study in the MTT assay was performed. A cell-friendly environment was generated as observed through MTT assay, with no significant differences in cytotoxicity. Immunohistochemical assay to verify VEGF expression was performed through an in vivo study. Samples containing VB with 2.5% Co demonstrated VEGF expression.
Considerações Finais/Final considerations/Consideraciones finales
Final considerations: The microparticles of Co-containing bioactive glasses with up to 2,5% CoO had VEGF expression and adequate structural properties, producing a friendly cellular environment and indicating that Co-containing sol-gel derived glasses are a potential candidate for application in tissue engineering and regenerative medicine.
Acknowledgments: The authors gratefully acknowledge PPGEM/UFMG and financial support from CNPq, CAPES and FAPEMIG.
Palavras-chave/Key words/Palabras clave
Key words: Bioactive glass, cobalt, angiogenesis.
ANDREIA GROSSI SANTOS DE LAIA, BRENO ROCHA BARRIONI, MARCOS AUGUSTO DE SÁ, THALITA MARCOLAN VALVERDE, PRICILA DA SILVA CUNHA, MARIVALDA DE MAGALHÃES PEREIRA